Overview
Type: Synthetic peptide
Form: Lyophilized Powder
ZnT4 (SLC30A4) Blocking Peptide (#BLP-ZT014) is the original antigen used for immunization during Anti-ZnT4 (SLC30A4) Antibody (#AZT-014) generation. The blocking peptide binds and ‘blocks’ Anti-ZnT4 (SLC30A4) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Anti-ZnT4 (SLC30A4) Antibody (#AZT-014), (1:500).
3-4. Anti-ZnT4 (SLC30A4) Antibody, preincubated with ZnT4 (SLC30A4) Blocking Peptide (BLP-ZT014).
Western blot analysis of rat small intestine lysate:1. Anti-ZnT4 (SLC30A4) Antibody (#AZT-014), (1:400).
2. Anti-ZnT4 (SLC30A4) Antibody, preincubated with ZnT4 (SLC30A4) Blocking Peptide (BLP-ZT014).
Western blot analysis of human LNCaP prostate adenocarcinoma cell line lysate (lanes 1 and 3) and human SH-SY5Y neuroblastoma cell line lysates (lanes 2 and 4):1-2. Anti-ZnT4 (SLC30A4) Antibody (#AZT-014), (1:500).
3-4. Anti-ZnT4 (SLC30A4) Antibody, preincubated with ZnT4 (SLC30A4) Blocking Peptide (BLP-ZT014).
Expression of ZnT4 in mouse hypothalamus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-ZnT4 (SLC30A4) Antibody (#AZT-014), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. ZnT4 immunoreactivity (green) appears in neuron outlines (arrows). B. Pre-incubation of the antibody with ZnT4 (SLC30A4) Blocking Peptide (BLP-ZT014), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)DEVSDEGLSRFNKLR, corresponding to amino acid residues 33 - 47 of rat SLC30A4 (Accession O55174).
Accession (Uniprot) Number O55174
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
