Cat #: BLP-ZT014
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized Powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- Peptide (C)DEVSDEGLSRFNKLR, corresponding to amino acid residues 33 - 47 of rat SLC30A4 (Accession O55174).
Accession (Uniprot) Number O55174
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Anti-ZnT4 (SLC30A4) Antibody (#AZT-014), (1:500).
3-4. Anti-ZnT4 (SLC30A4) Antibody, preincubated with ZnT4 (SLC30A4) Blocking Peptide (BLP-ZT014). - Western blot analysis of rat small intestine lysate:1. Anti-ZnT4 (SLC30A4) Antibody (#AZT-014), (1:400).
2. Anti-ZnT4 (SLC30A4) Antibody, preincubated with ZnT4 (SLC30A4) Blocking Peptide (BLP-ZT014). - Western blot analysis of human LNCaP prostate adenocarcinoma cell line lysate (lanes 1 and 3) and human SH-SY5Y neuroblastoma cell line lysates (lanes 2 and 4):1-2. Anti-ZnT4 (SLC30A4) Antibody (#AZT-014), (1:500).
3-4. Anti-ZnT4 (SLC30A4) Antibody, preincubated with ZnT4 (SLC30A4) Blocking Peptide (BLP-ZT014).
Last update: 02/01/2024