Overview
Alternative Name α-Conotoxin G1
Lyophilized Powder yes
Origin Synthetic peptide
MW: 1438 Da
Purity: >98% (HPLC)
Effective concentration 10 nM - 1 μM.
Sequence ECCNPACGRHYSC.
Modifications Disulfide bonds between Cys2-Cys7, Cys3-Cys13. Cys13 - Cysteine amide.
Structure
Molecular formula C55H80N20O18S4.
CAS No.: 76862-65-2
Activity α-Conotoxin GI inhibits α/δ site on mouse muscle-derived BC3H-1 nicotinic acetylcholine receptors (nAChR) expressed on postsynaptic membranes. Other site (α/γ site) on nicotinic receptors from Torpedo californica electric organ is also inhibited by α-Conotoxin GI1,2.
References-Activity
- Groebe, D.R. et al. (1995) Mol. Pharmacol. 48, 105.
- Groebe, D.R. et al. (1997) Biochemistry 36, 6469.
Accession number
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to two weeks at 4°C or three months at -20°C.
Our bioassay
Alomone Labs α-Conotoxin GI inhibits muscle fetal α1/β1/γ/δ nAChR heterologously expressed in Xenopus oocytes.A. Time course of α-Conotoxin GI (#STC-500) action on muscle α1/β1/γ/δ nAChR. Current amplitudes were plotted as a function of time. Membrane potential was held at –80 mV and oocytes were stimulated by exposure to 100 µM acetylcholine every 50 seconds. 100 nM (green) α-Conotoxin GI significantly inhibits the currents. B. Superimposed traces of α1/β1/γ/δ nAChR currents evoked by ACh (arrow) after application of control (black) and 100 nM (green) α-Conotoxin GI (taken from the recording in A).
References - Scientific background
- Gray, W.R. et al. (1981) The J. Biol. Chem. 256, 4734.
- Nishiuchi, Y. and Sakakibara, S. (1982) FEBS LETT. 148, 260.
- Guddat, L.W. et al. (1996) Biochemistry 35, 11329.
- Groebe, D.R. et al. (1995) Mol. Pharmacol. 48, 105.
- Groebe, D.R. et al. (1997) Biochemistry 36, 6469.
Scientific background
α-Conotoxin GI is a 13 amino acid peptidyl toxin isolated from the Conus geographus (Geography cone) venom1,2,3. It belongs to the Conotoxin A superfamily and reversibly blocks α/δ nicotinic ACh channel receptors (nAChR).
α-Conotoxin GI reversibly inhibits the high affinity α/δ site on mouse muscle-derived BC3H-1 receptor, a nAChR expressed on postsynaptic membranes4,5. Other low site (α/γ site) on nicotinic receptors from Torpedo californica electric organ is also inhibited by α-Conotoxin GI5.
Target α1/β1/δ/γ nAChR
Peptide Content: 100%
Lyophilized Powder
α-Conotoxin GI (#STC-500) is a highly pure, synthetic, and biologically active peptide toxin.
For research purposes only, not for human use
Last Update: 22/12/2024
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