Overview
Lyophilized Powder yes
Origin Modified synthetic peptide.
MW: 1924 Da
Purity: >95% (HPLC)
Form Lyophilized powder.
Effective concentration 1 – 2 µM.
Sequence GCCSDPRCAWRC.
Modifications Disulfide bonds between Cys2-Cys8, Cys3-Cys12. Cys12 - C-terminal amidation.
Label ATTO-590. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. ATTO-590 maximum absorption is 593 nm and maximum fluorescence is at 622 nm. The fluorescence is excited most efficiently in the 575-610 nm range. The extent of labeling is 1 molecule of dye per molecule α-Conotoxin ImI.
Activity α-Conotoxin ImI potently and specifically blocks mammalian neuronal nAChR (α3/β2 > α7 > α3/β4)1.
References-Activity
- McIntosh, J.M. et al. (1994) J. Biol. Chem. 269, 16733.
Accession number P50983.
Shipping and storage The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. Avoid exposure to light.
Solubility The product is lyophilized in 0.5 ml conical vial.
Centrifuge the vial before adding solvent (10,000 x g for 5 minutes). The lyophilizate may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed.
Soluble in pure water at high-micromolar concentrations (50 µM - 1 mM). For long-term storage in solution, it is recommended to prepare a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations at 10,000 x g for 5 minutes before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity. Avoid exposure to light.
Centrifuge the vial before adding solvent (10,000 x g for 5 minutes). The lyophilizate may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed.
Soluble in pure water at high-micromolar concentrations (50 µM - 1 mM). For long-term storage in solution, it is recommended to prepare a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations at 10,000 x g for 5 minutes before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity. Avoid exposure to light.
Storage of solutions Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.
Our bioassay
Alomone Labs α-Conotoxin ImI-ATTO Fluor-590 inhibits α3/β2 nAChRs expressed in Xenopus oocytes.A. Representative time course of α3/β2 current inhibition by 1.5 µM α-Conotoxin ImI-ATTO Fluor-590 (#C-290-AR). Currents were elicited every 50 sec by transient applications of 20 µM ACh, while membrane potential was held at -80 mV, and inhibited by 1.5 µM labeled toxin (green). B. Superimposed traces of α3/β2 currents evoked by ACh (arrow) after application of control (black) and of 1.5 µM α-Conotoxin ImI-ATTO Fluor-590 (green). Taken from the recording in A.
Alomone Labs α-Conotoxin ImI-ATTO Fluor-590 stains rat cerebellum.Rat cerebellum sections were incubated with 25 nM α-Conotoxin ImI-ATTO Fluor-590 (#C-290-AR). A. DAPI (blue) shows distribution outlines in the layers of the cerebellum (arrow). B. α-Conotoxin ImI-ATTO Fluor-590 stains (red) neuronal clusters (arrow) in the granule layer (G). There was weak nonspecific labeling of the molecular layer (MOL) and nearly no labeling in the white matter (WM) layer.
Alomone Labs α-Conotoxin ImI-ATTO Fluor-590 stains rat deep cerebellar nuclei.Rat deep cerebellar nuclei sections were incubated with 25 nM α-Conotoxin ImI-ATTO Fluor-590 (#C-290-AR). A. DAPI shows distribution outlines in the layers of the cerebellum. B. α-Conotoxin ImI-ATTO Fluor-590 stains cerebellar nuclei neurons (arrows).
References - Scientific background
- McIntosh, J.M. et al. (1994) J. Biol. Chem. 269, 16733.
- Johnson, D.S. et al. (1995) Mol. Pharmacol. 48, 194.
Scientific background α-Conotoxin ImI is a 12 amino acid peptidyl toxin isolated from Conus Imperialis venom1, which blocks mammalian neuronal nAChRs located on postsynaptic membranes. This toxin inhibits mammalian neuronal nAChRs (α3/β2 > α7 > α3/β4) in a voltage-independent manner and has no effect on nAChRs composed of α2/β2, α3/β2, α4/β2, α2/β4, α3/β4, or α4/β4 subunits1,2.
Target α3/β2 nAChR
Peptide Content: 100%
Lyophilized Powder
α-Conotoxin ImI-ATTO Fluor-590 (#C-290-AR) is a highly pure, synthetic, and biologically active conjugated peptide toxin.
We gladly take on collaboration projects. Please Contact Us.
For research purposes only, not for human use
Last Update: 25/11/2024
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