Overview
Type: Synthetic peptide
Form: Lyophilized powder
β1-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR023) is the original antigen used for immunization during Anti-β1-Adrenergic Receptor (extracellular) Antibody (#AAR-023) generation. The blocking peptide binds and ‘blocks’ Anti-β1-Adrenergic Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat lung (lanes 3 and 6) membranes:1-3. Anti-β1-Adrenergic Receptor (extracellular) Antibody (#AAR-023), (1:200).
4-6. Anti-β1-Adrenergic Receptor (extracellular) Antibody, preincubated with β1-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR023).
Expression of β1-Adrenoceptor in rat and mouse brainImmunohistochemical staining of rat dorsal raphe nucleus and mouse cerebellum using Anti-β1-Adrenergic Receptor (extracellular) Antibody (#AAR-023), (1:400). A. β1-Adrenoceptor staining (red) in rat dorsal raphe nucleus appears in neurons (horizontal arrows). C. β1-Adrenoceptor in mouse cerebellum staining (red) is detected in granule cells (horizontal arrows) and in the Purkinje cell layer (vertical arrow). B, D. Nuclear staining using DAPI as the counterstain (blue).
Properties
Sequence
- (C)RAESDEARR(S)YNDP, corresponding to amino acid residues 200-214 of rat ADRB1 (Accession P18090).
Accession (Uniprot) Number P18090
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
