Overview
Type: Synthetic peptide
Form: Lyophilized powder
β1-Syntrophin/SNTB1 Blocking Peptide (#BLP-PZ022) is the original antigen used for immunization during Anti-β1-Syntrophin (SNTB1) Antibody (#APZ-022) generation. The blocking peptide binds and ‘blocks’ Anti-β1-Syntrophin/SNTB1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:1,2. Anti-β1-Syntrophin (SNTB1) Antibody (#APZ-022), (1:500).
3,4. Anti-β1-Syntrophin (SNTB1) Antibody, preincubated with β1-Syntrophin/SNTB1 Blocking Peptide (#BLP-PZ022).
Western blot analysis of human HL-60 promyelocytic leukemia cell line lysate (lanes 1 and 3) and human HepG2 hepatocellular carcinoma cell line lysate (lanes 2 and 4):1,2. Anti-β1-Syntrophin (SNTB1) Antibody (#APZ-022), (1:500).
3,4. Anti-β1-Syntrophin (SNTB1) Antibody, preincubated with β1-Syntrophin/SNTB1 Blocking Peptide (#BLP-PZ022).
Properties
Sequence
- (C)EQVPESISNQKRGVK, corresponding to amino acid residues 99 -113 of mouse β1-syntrophin (Accession Q99L88).
Accession (Uniprot) Number Q99L88
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
