You want to know that your primary antibody binds the target protein it’s supposed to. Blocking peptides are one of …
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The protocols below are useful guides on how to work with Alomone Labs’ products.
For best results, we highly recommend that you calibrate certain experimental setups.
You want to know that your primary antibody binds the target protein it’s supposed to. Blocking peptides are one of …
detailsImprove antibody validation in IHC and ICC with a simple blocking peptide control. A blocking peptide is the original antigen …
detailsEnsure primary antibody specificity in western blotting with a simple blocking peptide control. A blocking peptide is the original antigen …
detailsA detailed protocol to take you through tissue processing and on to the direct method of IHC for floating sections …
detailsDetailed protocols to take you through tissue processing and on to indirect IHC methods for floating sections. Immunohistochemistry (IHC) allows …
detailsA clear protocol for using paraffin-embedded section with either DAB or fluorescence. Immunohistochemistry (IHC) protocols allow you to detect a …
detailsTarget multiple proteins simultaneously with these multiplex IHC protocols using antibodies from the same or different host species. Most immunohistochemistry …
detailsClear and detailed IHC steps for working with heart sections, from tissue preparation to staining. Immunohistochemistry (IHC) for fresh frozen …
detailsClear protocols for preparing cells for western blot with Laemmli buffer or mild detergents. Your sample preparation is critical for …
detailsEssential sample protocols to prepare tissues and brain synaptosomes for western blot. Sample preparation is a crucial first step in …
detailsA straightforward WB protocol from sample denaturation to blotting. A western blot (WB) lets you detect and evaluate the size …
detailsEasily run your western blot with large proteins like membrane channels. Western blotting high molecular weight (HMW) proteins, like sodium …
detailsDetailed cell preparation and direct and indirect ICC methods. Immunocytochemistry (ICC) allows you to locate a specific protein within or …
detailsStraightforward immunoprecipitation with Dynabeads® and a denaturing elution. Immunoprecipitation (IP) is a cornerstone technique for protein interaction and function studies, …
detailsDetailed steps to take you through cell preparation and on to indirect and direct methods of flow cytometry. Flow cytometry …
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