You want to know that your primary antibody binds the target protein it’s supposed to. Blocking peptides are one of …
detailsProtocols
The protocols below are useful guides on how to work with Alomone Labs’ products.
For best results, we highly recommend that you calibrate certain experimental setups.
Blocking Peptides
Blocking Peptide Protocols for Immunohistochemistry (IHC) and Immunocytochemistry (ICC)
Improve antibody validation in IHC and ICC with a simple blocking peptide control. A blocking peptide is the original antigen …
detailsBlocking Peptide Protocol for Western Blot (WB)
Ensure primary antibody specificity in western blotting with a simple blocking peptide control. A blocking peptide is the original antigen …
detailsImmunohistochemistry Protocols
Immunohistochemistry (IHC) Protocols for Frozen Sections: Direct Methods
A detailed protocol to take you through tissue processing and on to the direct method of IHC for floating sections …
detailsImmunohistochemistry (IHC) Protocols for Frozen Sections: Indirect Methods
Detailed protocols to take you through tissue processing and on to indirect IHC methods for floating sections. Immunohistochemistry (IHC) allows …
detailsImmunohistochemistry (IHC) Protocols for Paraffin-embedded Sections
A clear protocol for using paraffin-embedded section with either DAB or fluorescence. Immunohistochemistry (IHC) protocols allow you to detect a …
detailsImmunohistochemistry (IHC) Protocols for Frozen Sections: Multiplex Staining
Target multiple proteins simultaneously with these multiplex IHC protocols using antibodies from the same or different host species. Most immunohistochemistry …
detailsImmunohistochemistry (IHC) Protocol for Frozen Heart Sections
Clear and detailed IHC steps for working with heart sections, from tissue preparation to staining. Immunohistochemistry (IHC) for fresh frozen …
detailsSample Preparation Protocols for Western blot
Sample Preparation for Cell Lines
Clear protocols for preparing cells for western blot with Laemmli buffer or mild detergents. Your sample preparation is critical for …
detailsSample Preparation Protocols for Tissues
Essential sample protocols to prepare tissues and brain synaptosomes for western blot. Sample preparation is a crucial first step in …
detailsWestern Blot Protocols
Western Blot (WB) Protocol
A straightforward WB protocol from sample denaturation to blotting. A western blot (WB) lets you detect and evaluate the size …
detailsWestern Blot (WB) Protocol for High Molecular Weight (HMW) Proteins
Easily run your western blot with large proteins like membrane channels. Western blotting high molecular weight (HMW) proteins, like sodium …
detailsImmunocytochemistry Protocol
Immunocytochemistry (ICC) Protocols for Fixed or Live Cells: Indirect and Direct Methods
Detailed cell preparation and direct and indirect ICC methods. Immunocytochemistry (ICC) allows you to locate a specific protein within or …
detailsImmunoprecipitation Protocol
Immunoprecipitation using Dynabeads®
Straightforward immunoprecipitation with Dynabeads® and a denaturing elution. Immunoprecipitation (IP) is a cornerstone technique for protein interaction and function studies, …
detailsFlow Cytometry Protocol
Flow Cytometry Protocols for Live Cells: Indirect and Direct Methods
Detailed steps to take you through cell preparation and on to indirect and direct methods of flow cytometry. Flow cytometry …
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