Technical Service Guide: Immunohistochemistry Customer InformationFirst NameLast NamePhoneCompany/InstitutionEmailProduct InformationCat. #Product NameLot NumberPurchase DateProblem and Previous ExperienceWhat is the specific problem you are experiencing?Did this same vial of product work in the past? What were the results?Did other lots of this product work in the past? Which lots? What were the results?Please attach result images if relevantChoose an image ControlsHas the primary antibody (used in immunohistochemistry) been used successfully in other applications (Western Blot, Immunoprecipitation, Immunocytochemistry)?What positive and/or negative controls were done and what were the results?Did you use Alomone blocking peptide as a negative control? What were the results?Samples and FixationFrom which species (animal) was the sample?What type of sample was stained (frozen tissue, paraffin-embedded tissue)?What method of fixation was used (acetone, paraformaldehyde, methanol, other)?Was Antigen Retrieval performed? If so, by what method (heat treatment, enzyme digestion)?BlockingWhat were the blocking conditions (blocking solution, species of serum, length of incubation)?Primary AntibodyHow was the primary antibody reconstituted and stored?At what dilution(s) was the primary antibody used and what was the diluent?What were the incubation conditions (time, temperature) for the primary antibody?Secondary AntibodyFrom which company was the secondary antibody obtained and what type of secondary was being used (goat anti-rabbit, goat anti-guinea pig, etc.) and what secondary antibody conjugate was used (biotin, FITC, other)?At what dilution(s) was the secondary antibody used and what was the diluent?DetectionWhat method of detection was used (DAB, Immunofluorescence)?If a kit was used, was the kit used successfully with other primary antibodies?In order to process your request as soon as we can, please attach result images below:Choose File Alomone Labs will process your data following our privacy policy. This will include sending you updates about us, our products, and related scientific information we believe would be of interest to you. You can manage your preferences or unsubscribe by clicking the link at the bottom of every email we send. Submit
