A single antibody that saves valuable resources – and enables immediate results!
Working with Alomone Labs ATTOTM-conjugated antibodies provides significant value. Its direct fluorescent probe differentiates without the need for secondary antibodies, providing a visual picture of your results, instantly. Ideal for multiplex staining with the same species/host antibodies.
Now you can use just one effective antibody, while saving time and resources.
That’s the true color of success
ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescent quantum yield, and high photo-stability. They are analogous to Alexa dyes and are comparable to any fluorescent technology.
|
Label |
Fluorescence |
Spectra |
Alternative to |
|---|---|---|---|
|
ATTO-488 |
Green |
λex 501 nm; λex 523 nm in 0.1 M phosphate pH 7.0 |
FITC, Alexa-488 |
|
ATTO-550 |
Orange |
λex 554 nm; λex 576 nm in 0.1 M phosphate pH 7.0 |
TAMRA, Cy3, |
|
ATTO-594 |
Red |
λex 601 nm; λex 627 nm in 0.1 M phosphate pH 7.0 |
Texas Red, |
|
ATTO-633 |
Far red |
λex 629 nm; λex 657 nm in 0.1 M phosphate pH 7.0 |
Alexa-647, Cy5 |
|
|
ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. |
|
|
ATTO-550. Maximum absorption 554 nm; maximum fluorescence 576 nm. The fluorescence is excited most efficiently in the 540 – 565 nm range. This label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. |
|
|
ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 – 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. |
|
|
ATTO-633 belongs to a new generation of fluorescent labels for the red spectral region. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. The label is moderately hydrophilic. Its fluorescence is excited most efficiently in the range 610 – 645 nm. This dye is highly suitable for direct flow cytometry (FACS) using the He:Ne laser. |
Within our antibody portfolio, we gladly take on special requests for customized antibody labeling/conjugation.