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Overview
Anti-DLGAP1/GKAP Antibody (#APZ-041) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize DLGAP1 from human, mouse, and rat samples.
Applications: IF, IHC, WB
Reactivity: H, M, R
Alternative Name DLG associated protein 1, Discs large homolog associated protein 1, Guanylate kinase-associated protein, SAP90/PSD-95-associated protein 1, SAPAP1
Accession (Uniprot) Number P97836
Gene ID 65040
For research purposes only, not for human use
Applications
Citations (8)
Citations
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Properties
Immunogen
- Peptide (C)KISNEHSPKLQIRSH, corresponding to amino acid residues 397-411 of rat DLGAP1 (Accession P97836). Intracellular.
Homology Mouse, human – identical.
RRID AB_2756784.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Standard quality control of each lot Western blot analysis.
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Scientific Background
Scientific Background
Scientific background
DLGAP1/GKAP (discs large homolog associated protein 1/guanylate kinase associated protein) is a member of the DLGAP family, scaffold proteins localized to the postsynaptic density. The protein is involved in signaling to and from glutamate receptors, it serves as a key adaptor protein of NMDA receptors, involved in learning and memory and synaptic plasticity in the central nervous system1,2.
DLGAP family comprises five conserved proteins, DLGAP1–DLGAP5. DLGAP1-DLGAP4 proteins interact directly with both DLG and SHANK through their multiple domains. DLGAP1–4 proteins are implicated in several neurological disorders including schizophrenia, autism, obsessive compulsive disorder and cerebellar ataxia1.
DLGAP1 is highly expressed in the brain; Dlgap1 mRNA is detected in the cortex, hippocampus, olfactory bulb, striatum and thalamus1.
References
- Rasmussen, A.H. et al. (2017) Mol. Brain 10, 43.
- Li, L. et al. (2018) Cancer Cell 33, 736.
Last Update: 12/03/2025
