Name: Recombinant human Neurotrophin-3 (NT-3) protein
Brand: Alomone Labs
SKU: N-260

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Overview

Cat #: N-260

Lyophilized Powder yes

Sterile & Endotoxin Free yes

Origin Recombinant, E. coli

MW: 27.2 kDa

Endotoxin Level <0.1 EU per 1 µg of the protein by the LAL method.

Purity: >98% (HPLC)

Form Lyophilized from a 0.2 µm filtered solution.

Sequence YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT

Structure

Activity NT-3 is involved in many biological outcomes including strengthening synaptic connections to motoneurons¹ and serving as an anti-inflammatory factor to suppress microglial activation² among many other cellular functions. NT-3 biological effects are mediated by two receptors: TrkC, which is specific for NT-3 and p75^NTR, which binds all neurotrophins³.

References-Activity

Arvanian, V.L. et al. (2003) J. Neurosci. 23, 8706.
Tzeng, S.F. and Huang, H.Y. (2003) J. Cell Biochem. 90, 227.
Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35.

Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Solubility Sterile water at a concentration of at least 1 μg/0.1 ml. BSA (0.1 mg/ml) should be added for more diluted solutions. Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.

Storage of solutions Up to one week at 4°C or four-six weeks at -70°C.

Our bioassay

Alomone Labs Recombinant human Neurotrophin-3 (NT-3) protein mediates neurite outgrowth in TrkC-transfected PC12 cells.
One day post-transfection, cells were stimulated with 30 ng/ml Recombinant human Neurotrophin-3 NT-3 protein (#N-260). Neurite outgrowth was visualized by light microscopy 5 days after treatment.
Alomone Labs Recombinant human Neurotrophin-3 (NT-3) protein induces ERK1/2 MAPK phosphorylation in TrkC transfected PC12 cells.
Three days post transfection, cells were serum deprived for 3 hours and then challenged with varying concentrations of Recombinant human Neurotrophin-3 (NT-3) protein (#N-260) for 8 minutes. Cell extracts were prepared, resolved by SDS-PAGE and probed with anti-phospho-ERK1/2.

Scientific background

The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and PNS.¹

Of all the neurotrophins, Neurotrophin-3 (NT-3) shows the highest expression levels during the perinatal development, with most prominent expression levels in the hippocampus, the neocortex and the cerebellum.² Release of endogenous NT-3 was observed from cultures of embryonic hippocampus following high K⁺ induced depolarization or stimulation with activity dependent neurotrophic factor (ADNF).³

The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF.⁴ The rat and human forms of NT-3 are 96% homologous.

NT-3 has been shown to strengthen synaptic connections to motoneurons in the neonatal rat,⁵ to serve as an anti-inflammatory factor to suppress microglial activation,⁶ to play a critical role in regulating T helper 1/T helper 2 cell balance⁷and to modify potassium currents in isolated inner hair cells from guinea pig cochlea.⁸

The biological effects of NT-3 are mediated by two receptors: TrkC, which is specific for NT-3 and p75^NTR, which binds all the neurotrophins.⁹

Peptide Content: 100%

Lyophilized Powder

For research purposes only, not for human use

Last Update: 07/05/2024

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Recombinant human Neurotrophin-3 (NT-3) protein

Overview

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Scientific Background

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