Overview
Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.
Our Rabbit IgG Isotype Control-FITC (#RIC-001-F), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to FITC.
For research purposes only, not for human use
Applications
Citations
Citations
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- Wang,p. et al. (2023) Cell Stem Cell. 10.1016.
Specifications
Properties
Purity Serum from non-immunized rabbits was pooled and the IgG fraction was purified on a Protein A column.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Specificity The IgG fraction was purified form non-immunized rabbits.
Label Fluorescein isothiocyanate (FITC)
Standard quality control of each lot Flow cytometry
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Working dilution The isotype control antibody should be used at the exact same dilution of the experimental antibody
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Last Update: 12/03/2025
