Overview
Type: Synthetic peptide
Form: Lyophilized powder
STIM2 Blocking Peptide (#BLP-CC064) is the original antigen used for immunization during Anti-STIM2 Antibody (#ACC-064) generation. The blocking peptide binds and ‘blocks’ Anti-STIM2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1, 3) and rat RBL basophilic leukemia cell (lanes 2, 4) lysates:1. Anti-STIM2 Antibody (#ACC-064), (1:200).
2. Anti-STIM2 Antibody, preincubated with STIM2 Blocking Peptide (#BLP-CC064).
Expression of STIM2 in rat lungImmunohistochemical staining of paraffin embedded rat lung sections using Anti-STIM2 Antibody (#ACC-064), (1:100). Staining is present in the respiratory epithelium of the bronchiole (red) as well as in the pneumonocytes of the alveolar wall (blue). Color reaction was obtained with SuperPicture HRP-conjugated polymer (Zymed) followed by DAB. Hematoxilin is used as the counterstain.
Stromal interaction molecule 2 (STIM2) in rat subventricular zone (SVZ) regionFree-floating frozen brain sections from a paraformaldehyde-perfused rat were incubated with rabbit anti-STIM2 antibody (#ACC-064, 1:300), followed by goat anti-rabbit secondary antibody conjugated to Alexa Fluor® 488 (green). A. STIM2 immunoreactivity was observed in the SVZ region (arrows). B. Pre-incubation of the primary antibody with the STIM2 Blocking Peptide (#BLP-CC064) suppressed the staining. Nuclear staining with DAPI (blue). LV = lateral ventricle.
Stromal interaction molecule 2 (STIM2) in rat medial septum (MS) regionFree-floating frozen brain sections from a paraformaldehyde-perfused rat were incubated with rabbit anti-STIM2 antibody (#ACC-064, 1:300), followed by goat anti-rabbit secondary antibody conjugated to Alexa Fluor® 488 (green). A. STIM2 immunoreactivity was observed in the medial septum region (arrows). B. Pre-incubation of the primary antibody with the STIM2 Blocking Peptide (#BLP-CC064) suppressed the staining. Nuclear staining with DAPI (blue).
Stromal interaction molecule 2 (STIM2) in mouse nucleus basalis (NBM) regionFree-floating frozen brain sections from a paraformaldehyde-perfused mouse were incubated with rabbit anti-STIM2 antibody (#ACC-064, 1:300), followed by goat anti-rabbit secondary antibody conjugated to Alexa Fluor® 488 (green). A. STIM2 immunoreactivity was observed in the NBM region (arrows). B. Pre-incubation of the primary antibody with the STIM2 Blocking Peptide (#BLP-CC064) suppressed the staining. Nuclear staining with DAPI (blue).
Properties
Sequence
- SAEKQWEVPDTASEC, corresponding to amino acid residues 583-597 of human STIM2 (Accession Q9P246).
Accession (Uniprot) Number Q9P246
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
